Chromatography techniques

 

 

High Performance Liquid Chromatography (HPLC) / Ultra Performance liquid Chromatography (UPLC)

 

Gas Chromatography (GC)

 

Preparative Liquid Chromatography

 

Ion Chromatography (IC)

 

Amino Acid Analysis by Ninhydrin-positive Substances (AAA by NPS)

 

 

 

 

 

High Performance Liquid Chromatography (HPLC) / Ultra Performance Liquid Chromatography (UPLC)

 

 

HPLC is a widely used chromatographic technique in which a liquid mobile phase is used to separate, identify, and quantify the different components of a sample. The technique relies on pumps to pass pressurised liquid solvents through a column filled with solid absorbent materials. HPLC is commonly used in the analysis of pharmaceuticals, food and beverages, environmental samples, and many other applications. HPLC provides several advantages including high sensitivity and its ability to be applied to different compounds and handle complex mixtures.

 


UPLC is a more advanced and high-resolution form of HPLC that uses smaller particle sizes in the column packing material, which allows for faster separations and improved sensitivity. This technique is commonly used in the analysis of complex mixtures, such as proteins and peptides.

Equipment

 

  • Agilent 1100 to 1290 series with UV, DAD, RID, FLD, ELSD and MS (Binary & Quaternary systems)
  • USP& EP qualified

 

  • HPLC

    Life science capabilities and applications

    • Product development support (Early stage to CMC)
    • Compound screening
    • Release testing
    • Assay development
    • Dissolution testing
    • Extensive experience in a variety of sample types - oral dose forms (capsules, Tablets, Suspensions), suppositories, dermal patches, Topical formulations.
    • Extensive experience in small molecules & Biopharmaceuticals including protein characterisation.
    • Stability studies
    • Investigatory analysis including impurity analysis
    • Comparative analysis
    • Method Validation
    • Method Verification
    • Method Transfer
    • Pharmacopeial Testing
    • Biopharmaceutical Testing (RP, SEC and IEX)
    • HPLC method development and trouble shooting
    • USP  & EP qualified equipment
    • All methods and testing performed under GMP requirements and FDA & MHRA inspected.

 

Gas Chromatography (GC)

 

 

GC is a technique that uses a gaseous mobile phase to separate the different components of a sample. The sample is vaporised and injected into a column packed with a stationary phase, the components are then separated based on their differential interaction with the stationary phase. RSSL has GCs equipped with Headspace Autosamplers, an automated device that extracts the volatile compounds and injects the extracted vapour into the GC system for separation and analysis. GC is commonly used in the analysis of volatile organic compounds, such as those found in pharmaceutical products, food flavours and beverages, and cosmetics. 

Equipment

 

 

  • Agilent Gas Chromatograph (with Headspace Autosampler)
  • Perkin Elmer Gas Chromatograph (with Headspace Autosampler)
  • Flame Ionization Detector (FID)
  • Thermal Conductivity Detector (TCD)
  • Electron Capture Detector (ECD)
  • Nitrogen Chemiluminescence Detector (NCD)
  • Mass Spectrometer Detector (MSD)

 

  • GC

    Life science capabilities and applications

    • Extensive Experience in Pharmacopeial methods including:
      • Ethylene Oxide and Dioxan (USP <228> & EP 2.4.25)
      • Residual Solvents (USP <467> and EP 2.4.2.4)
      • Fatty Acid Composition (USP <401> & EP 2.4.22)
    • Raw Material testing including identification and purity analysis
    • Pharmaceutical Release testing
    • Residual solvent analysis
    • Development Material & Stability Study Sample testing
    • Active Pharmaceutical Ingredients testing
    • Method Validation
    • Method Verification
    • Method Transfer
    • GC/GC-Headspace method troubleshooting, optimisation and/or development.
    • USP  & EP qualified equipment
    • All methods and testing performed under GMP requirements and FDA & MHRA inspected.

 

 

Preparative Liquid Chromatography

 

Preparative Liquid Chromatography (LC) is a chromatographic technique used to separate, isolate and purify large amounts of a target compound from a complex mixture. Unlike analytical LC, which is used to analyse small amounts of a sample, preparative LC is used to produce large quantities of a purified compound for subsequent use in research or industry. RSSL has tandem Mass Spectrometry (MS/MS) and Nuclear Magnetic Resonance (NMR) capabilities which can be used in conjunction with Prep-HPLC.

 

 

Equipment

 

  • Agilent 1290 Infinity II Binary Prep-HPLC system
    • Coupled with UV-DAD (Prep-Scale)
    • Coupled with UV-DAD (Analytical-Scale)
    • 1290Infinity II Fraction Collection capacity
  • Waters 2525 with PDA 2998 & Fraction collection 2767.
  • Prep LC

    Life science capabilities and applications

    • Bespoke impurity isolation from traditional small molecules to biopharmaceutical compounds, scaled with ease from analytical concentrations up to gram quantities.
    • Isolation and concentration of target impurities.
    • Purification
    • Extensive experience in upscaling analytical methods to prep-scale
    • Purification of small molecules and Bio-Pharmaceutical products

 

 

 

Ion Chromatography (IC)

 

A chromatographic technique used to separate and quantify ions based on their charge and affinity for a stationary phase. IC has several important applications that contribute to quality control, regulatory compliance, and research. This technique is suitable for application to a wide range of matrices, of which it is commonly used in the analysis of inorganic and organic ions, such as those found in water samples, pharmaceuticals, and food and beverage products. 

 

 

Equipment

 

  • Thermo Scientific/Dionex ICS5000 to ICS6000 series (quaternary pumps) with conductivity detection (CD) and pulsed amperometric detection (PAD).  
  • IC

    Life science capabilities and applications

    • Counterion determination
    • Investigation into low-level nitrite/nitrates within nitrosamine pathways.
    • Carbohydrate profiling (key in characterisation of glycoproteins)
    • Product Development (Early stage to CMC) including ion interaction
    • Release testing
    • Stability testing including ionic degradation and strength stability
    • Investigatory analysis
    • Comparative analysis
    • Method Validation
    • Method Transfer
    • Method Verification
    • Method development and troubleshooting
    • Pharmacopeial Testing
    • Expediated turnaround times available
    • USP & EP qualified equipment
    • All methods and testing performed under GMP requirements and FDA & MHRA inspected.

 

Amino Acids Analysis by Ninhydrin-Positive Substances (AAA by NPS)

 

Ninhydrin is a chemical compound that reacts with primary amino groups in amino acids and proteins, resulting in a colour change that can be used for detection and quantification. This reaction is commonly utilized in amino acid analysis, particularly in chromatographic techniques like thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). Ninhydrin-based amino acid analysis is a widely used technique in biochemistry and protein research, allowing for the accurate quantification and identification of amino acids in complex mixtures, this is crucial for understanding protein composition and structure.

 

 

Equipment

 

  • Thermo Scientific ICS6000 series, coupled with Pinnacle PCX post column derivatisation unit, with UV/visible detection.
  • AAA Dionex ICS5000

    Life science capabilities and applications

    • Pharmacopeial Testing
    • Method pre-verified for use for the following EP monographs: Serine, Valine, Proline, Leucine, Isoleucine, Histidine, Arginine, Lysine, Glycine, Alanine, Tyrosine, Cysteine, Phenylalanine, Aspartic acid, Tryptophan, as free acid or salt forms.
    • Release testing support
    • Stability testing
    • Investigatory analysis including determination of impurities
    • Comparative analysis (quantitative and qualitative)
    • Method Validation
    • Method Transfer
    • Method Verification
    • Method development and troubleshooting
    • Expediated turnaround times available
    • USP& EP qualified equipment’s
    • All methods and testing performed under GMP requirements and FDA & MHRA inspected.